BIOL-375 Molecular Genetics
Bioinformatics | Biology | Genetics | Genomics | Molecular Genetics
Description, Abstract, or Artist's Statement
ABSTRACT. This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2874 and Mrub_1349. We predict that Mrub_2874 encodes the enzyme ribulose-5-phosphate 3-epimerase (DNA coordinates 2912530..2913204 on the reverse strand), which is the first step of the pentose phosphate pathway (KEGG map number 00030). It catalyzes the conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate. The E. coli K12 MG1655 ortholog is predicted to be b3386, which has the gene identifier rpe. We predict that Mrub_1349 encodes the enzyme ribose-5-phosphate isomerase (DNA coordinates 1381292..1381978 on the reverse strand), which is also the first step of the pentose phosphate pathway (KEGG map number 00030). It catalyzes the conversion of D-ribulose 5-phosphate to D-ribose 5-phosphate. The E. coli K12 MG1655 ortholog is predicted to be b2914 and b4090, which have the gene identifiers rpiA and rpiB respectively. The comparison of Mrub_2875 to b3386 and the comparison of Mrub_1349 to b2914 were conclusive, but the data was inconclusive when comparing Mrub_1349 to b4090.
Augustana Digital Commons Citation
Murad, Samantha and Scott, Dr. Lori. "Mrub_2874 is homologous to b3386 and Mrub_1349 is homologous to b2914, but Mrub_1349 is not homologous to b4090" (2016). Meiothermus ruber Genome Analysis Project.