Course

BIOL 375 Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

2017

Disciplines

Bioinformatics | Biology | Genomics | Microbiology | Molecular Genetics

Description, Abstract, or Artist's Statement

ABSTRACT. This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_0173, Mrub_0125, and Mrub_ 2910. We predict that Mrub_0173 encodes the enzyme phosphoglycerate dehydrogenase (DNA coordinates 152982 ... 154347), which is the 1st step of the serine biosynthesis pathway (KEGG map number 00680). It catalyzes the conversion of NAD+ + 3-phospho-D-glycerate → NADH H+ + 3-phospho-hydroxypyruvate. The E. coli K12 MG1655 ortholog is predicted to be b2913, which has the gene identifier serA. We predict that Mrub_0125 encodes the enzyme phosphoserine phosphatase (DNA coordinates 103483 ... 104136), which is the 3rd step of the serine biosynthesis pathway (KEGG map number 00680). It catalyzes the conversion of H2O + 3-phosopho-L-serine → phosphate + L-serine. The E. coli K12 MG1655 ortholog is predicted to be b4388, which has the gene identifier serB. We predict that Mrub_2910 encodes the enzyme glycine hydroxymethyltransferase (DNA coordinates 2946484 ... 2947716), which is the 1st step of the glycine biosynthesis and degradation pathway (KEGG map number 00680). It catalyzes the conversion of 5,10-methylene-tetrahydrofolate + H2O + glycine ↔tetrahydrofolate + L-serine. The E. coli K12 MG1655 ortholog is predicted to be b2551, which has the gene identifier glyA.

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