BIOL-375 Molecular Genetics
Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2507 and Mrub_2888. We predict that Mrub_2507 encodes the enzyme pyridoxal kinase (DNA coordinates 2555521..2556402), which is in the Vitamin B6 Metabolism pathway (KEGG map number 00750). It catalyzes the conversion of pyridoxine, pyridoxamine, or pyridoxal to pyridoxine 5’-phosphate, pyridoxamine 5’-phosphate, or pyridoxal 5’-phosphate respectively. The E. coli K12 MG1655 ortholog is predicted to be b1636, which has the gene identifier pdxY. We predict that Mrub_2888 encodes the enzyme pyridoxal 5’-phosphate synthase (DNA coordinates 2924422..2925054), which is in the Vitamin B6 Metabolism pathway (KEGG map number 00750). It catalyzes the conversion of pyridoxine 5’-phosphate or pyridoxamine 5’-phosphate to pyridoxal 5’-phosphate. The E. coli K12 MG1655 ortholog is predicted to be b1638, which has the gene identifier pdxH.
Augustana Digital Commons Citation
Bernardi, Adam and Scott, Dr. Lori. "Bioinformatics Comparison of M. ruber Mrub_2507 to E. coli pdxK/b1636 and M. ruber Mrub_2888 to E. coli pdxH/b1638 to Determine the Orthologous Nature" (2016). Meiothermus ruber Genome Analysis Project.