BIOL-375 Molecular Genetics
Bioinformatics | Biology | Genetics | Molecular Biology | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_1907 and Mrub_1844. We predict that Mrub__1907 encodes the enzyme ketol-acid reductoisomerase (DNA coordinates 1966630..1967649 on the reverse strand), which is the fourth step of the L-isoleucine pathway (from threonine) (KEGG map number 00290). It catalyzes the conversion of (R)-3- Hydroxy-3-methyl-2-oxopentanoate to (R)-2-3 Dihydroxy-3-methylpentanoate. The E. coli K12 MG1655 ortholog is predicted to be b3774, which has the gene identifier ilvC. We predict that Mrub__1844 encodes the enzyme dihydroxy-acid dehydratase (DNA coordinates 1901362..1903026) on the reverse strand), which is the fifth step of the L-isoleucine pathway (from threonine) (KEGG map number 00290). It catalyzes the conversion of (R)-2-3 Dihydroxy-3-methylpentanoate to (S)-3-Methyl-2-oxopentanoate. The E. coli K12 MG1655 ortholog is predicted to be b3771, which has the gene identifier ilvD.
Augustana Digital Commons Citation
Hernandez, Felipe A. and Scott, Dr. Lori. "Genomic Analysis of Meiothermus ruber Mrub_1907 and Meiothermus ruber Mrub_1844 with Potential Ortholog Escherichia coli b3774 IlvC and Escherichia coli b3771 IlvC Gene through Bioinformatics" (2016). Meiothermus ruber Genome Analysis Project.