BIOL-375 Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date



Biochemistry | Bioinformatics | Biology | Evolution | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2642, Mrub_1054, and Mrub_1059.

We predict that Mrub_2642 encodes the enzyme methionine adenosyltransferase (DNA coordinates [2677251…2678426] on the reverse strand), the first step of the methionine degradation pathway (KEGG map number 00270). Methionine adenosyltransferase catalyzes the conversion of the substrates, ATP, L-methionine, and water, to yield the products S-adenosyl-L-methionine (SAM), inorganic phosphate, and diphosphate. Mrub_1054 encodes adenosylhomocysteine nucleosidase (DNA coordinates [1054556…1055239] on the reverse strand]), the enzyme in the third step of this pathway, which catalyzes the conversion of SAM and water to produce S-ribosyl-L-homocysteine and adenine. In the fourth step of this pathway, Mrub_1054 encodes the enzyme S-ribosylhomocysteine lyase (DNA coordinates [1060000…1060485] on the reverse strand), and catalyzes the formation of autoinducer II (AI-2) by cleavage of S-ribosyl-L-homocysteine, leaving L-homocysteine as a product. The E. coli K12 MG1655 orthologs are predicted to be b2942, b0159, and b2687, which have the gene identifiers metK, mtn, and luxS.