BIOL-375 Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date



Biochemistry | Bioinformatics | Biology | Evolution | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_1867, Mrub_1868, and Mrub_1869. We predict that Mrub_1867 (DNA coordinates 1927237..1927527 on the reverse strand), Mrub_1868 (DNA coordinates 1927524..1928123 on the reverse strand), and Mrub_1869 (DNA coordinates 1928248..1928781 on the reverse strand) are subunits of the NADH: ubiquinone oxidoreductase complex (KEGG map number 00190). This complex catalyzes the translocation of H+ across the cytoplasmic membrane and transfers electrons from NADH to ubiquinone during oxidative phosphorylation. The respective E. coli K12 MG1655 orthologs are predicted to be b2279, b2280, and b2281 which have the gene identifiers NuoK, NuoJ, and NuoI.