Course

BIOL-375: Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

2018

Disciplines

Biodiversity | Bioinformatics | Biology | Biophysics | Cellular and Molecular Physiology | Genetics | Genetics and Genomics | Genomics | Molecular Biology | Molecular Genetics | Structural Biology

Description, Abstract, or Artist's Statement

In this project we investigated the biological function of the genes Mrub_1751, Mrub_1752 and Mrub_1753 (KEGG map number 02010). We predict these genes encode components of a D-xylose ATP Binding Cassette (ABC) transporter: 1) Mrub_1752 (DNA coordinates 1809004-1810224 on the forward strand) encodes the permease component (aka transmembrane domain), predicted to be an ortholog and 2) Mrub_1753 (DNA coordinates 1810227-1811000 on the forward strand) encodes the ATP-binding domain (aka nucleotide binding domain); and 3) Mrub_1751 (DNA coordinates 1807855-1808892 on the forward strand) encodes the solute binding protein. The ABC-transporter for M. ruber to transport D-xylose is homologous with the transporter found in E. coli, allowing for the transport of D-xylose into the cell. Of interest is the finding that Mrub_1753 and E. coli xylG appear to be partly embedded in the cell membrane, unlike most ATP-binding domains. This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT).

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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