BIOL-375: Molecular Genetics

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Student Paper

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Biochemistry | Bioinformatics | Biology | Cellular and Molecular Physiology | Comparative and Evolutionary Physiology | Computational Biology | Evolution | Genetics | Genomics | Integrative Biology | Molecular Biology | Molecular Genetics | Structural Biology

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In this project we investigated the biological function of the genes Mrub_1325, Mrub_1326, Mrub_1327, and Mrub_1328 (KEGG map number 02010). We predict these genes encode components of a Branched Chain Amino Acid ATP Binding Cassette (ABC) transporter: 1) Mrub_1325 (DNA coordinates 1357399-1358130 on the reverse strand) encodes the ATP binding domain; 2) Mrub_1326 (DNA coordinates 1358127-1359899 on the reverse strand) encodes the ATP-binding domain and permease domain; 3) Mrub_1327 (DNA coordinates 1359899-1360930 on the reverse strand) encodes a permease domain; and 4)Mrub_1328 (DNA coordinates 1711022-1712185 on the reverse strand) encodes the substrate binding domain. This system is not predicted to have a solute binding protein, which is a component of most ABC transporters. This transport system is found in E. coli K12 MG1655, the predicted orthologs of Mrub_1325, Mrub_1326, Mrub_1327, and Mrub_1328, livF, livG, livH, and livK respectively, are b3454, b3455, b3457, and b3458, form a livFGHK operon encoding an ABC transporter for branched chain amino acid transport. Mrub_1326 is likely a fused protein of both livG and livM suggesting it may also be orthologous to b3456. This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT).

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This work is licensed under a Creative Commons Attribution 4.0 License.