Senior Inquiry

Document Type

Student Paper

Publication Date

Spring 5-2018


Bacteriology | Biochemistry | Bioinformatics | Biology | Cell Biology | Evolution | Genetics | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of Escherichia coli and Meiothermus ruber proC genes using the complementation assay. In this research project, mutants of varying severity to the functional state of the protein were developed. The results showed that two or more amino acid deletions reduced or eliminated ProC function. Amino acid substitutions, on the other hand, were not severe enough to impact ProC function. Double and triple mutants could be distinguished under the experimental conditions. Additionally, a difference in the growth pattern of M. ruber ProC (nonmutated or mutated) as compared to the comparable nonmutant or mutant state in E. coli, respectively, was observed. This is attributed to M. ruber protein's adaptability to function at higher temperatures.