Course

BIOL-375: Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

Winter 2-13-2019

Disciplines

Biology | Computational Biology | Genetics | Genomics | Immunology and Infectious Disease | Molecular Genetics

Description, Abstract, or Artist's Statement

This research is part of the Meiothermus ruber genome annotation project which aims to predict gene function with various bioinformatics tools. We investigated the function of Mrub_3019, which encodes the CasA protein involved in the multi-subunit effector complex for the CRISPR-Cas immunity system and predicted it to be an ortholog of E. coli K12 MG1655 b2760 (casA). We predicted that Mrub_3019 encodes the protein CasA, which is involved in PAM recognition of CRISPR interference pathway. Foreign DNA will bind to CasA, which signals Cas3 for helicase-mediated DNA degradation. Our hypothesis is supported by low E-values for pairwise alignment in NCBI BLAST, Pfam, and TIGRfam. Both proteins were predicted to be non-transmembrane-embedded, and in close proximity to Cas3 in the Type I-E CRISPR-Cas complex. Finally, both revealed several amino acids that were identical in the Pfam domain hit (PF0481) for the CRISPR_Cse1 family.

Key words: Meiothermus ruber, genome, bioinformatics, annotation, CRISPR-Cas, prokaryotic defense, Mrub_3019, b2760, ortholog, casA

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Additional Files:

Heiland_takehomemidterm_Mgenetics (1).pdf (106 kB)
Adding histidine tag to E. coli and M. ruber proC

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