BIOL-375: Molecular Genetics
Bioinformatics | Biology | Computational Biology | Genomics | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We investigated the biological function of gene Mrub_3013, which we hypothesize is orthologous to b2755 in E. coli K12 MG1655 (a.k.a. Cas1). We investigated the biological function of a gene with the M. ruber locus tag of Mrub_3013, which we hypothesize is a component of the CRISPR-Cas prokaryotic defense system in M. ruber. We predict that Mrub_3013 (DNA coordinates 3,053,978-3,054,940) encodes the protein Cas1 which as part of the CRISPR-Cas system, selects and cuts the foreign DNA to make the spacer using the Cas1-Cas2 complex to restrict the size for recognition of an appropriate protospacer. Our hypothesis is supported by identical hits for Mrub_3013 and b2755 to the KEGG, TIGRfam, CDD and PDB databases, as well as a low E-value for a pairwise NCBI BLAST comparison. Both protein products are predicted to be localized to the cytoplasm due to TMHMM probabilities, PRED-TMBB probabilities, and PSORT-B data scores.
Augustana Digital Commons Citation
Butcher, Laura and Scott, Dr. Lori. "M. ruber Mrub_3013 is Orthologous to E. coli b2755" (2019). Meiothermus ruber Genome Analysis Project.
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Additional Files:Adding Histidine Tag to E. coli and M. ruber proC.pdf (73 kB)
Adding Histidine Tag to E. coli and M. ruber proC