BIOL-375: Molecular Genetics
Bioinformatics | Biology | Computational Biology | Genomics | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We investigated the biological function of the gene Mrub_3014, which we hypothesize is a component of the CRISPR-Cas prokaryotic defense system. We predict that Mrub_3014 (DNA coordinates 3054943..3055575) encodes CRISPR-associated protein Cse3/case which function as an endonuclease. Our hypothesis is supported by identical hits for Mrub_3014 and b2756 to the KEGG, Pfam, TIGRfam, CDD and PDB databases, as well as a low E-value for a pairwise NCBI BLAST comparison. Both protein products are predicted to be localized to the cytoplasm. Finally, both proteins share numerous highly conserved amino acids, when compared to the consensus sequence of Pfam domains (PF08798 and PF08798).
Augustana Digital Commons Citation
Abdelkarim, Samir and Scott, Dr. Lori. "Mrub_3014 is orthologous to b2756" (2019). Meiothermus ruber Genome Analysis Project.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License