Course

BIOL-375: Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

2019

Disciplines

Bioinformatics | Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We investigated the biological function of mrub_3013 and the nature of its relationship with mrub_1477 and mrub_0224. We hypothesized that mrub_3013 is orthologous to b2755 in E. coli K12 MG1655 (a.k.a. cas1). We predict that mrub_3013 encodes the enzyme Cas1, which is involved in spacer acquisition in the CRISPR-Cas prokaryotic defense system. Our hypothesis is supported by identical hits for b2755, mrub_3013, mrub_1477, and mrub_0224 from the CDD and Pfam databases and highly similar hits from the TIGRfam and PDB databases, as well as low E-values for pairwise NCBI Protein Blast comparisons. We predicted that mrub_3013 would have a paralogous relationship with mrub_1477 and mrub_0224. While mrub_3013, mrub_1477, and mrub_0224 are all clearly cas1 genes, they do not appear to be evolutionarily related and may not be true paralogs.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Additional Files:

Midterm Paper.pdf (70 kB)
Adding a histidine tag to E. coli and M. ruber proC.

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