Course
BIOL-375: Molecular Genetics
Document Type
Student Paper
Publication Date
2018
Disciplines
Biology | Molecular Genetics
Description, Abstract, or Artist's Statement
In this project we investigated the biological function of the genes Mrub_2379, Mrub_2378, Mrub_2374, Mrub_2376, and Mrub_2377 (KEGG map number 02010). We predict these genes encode components of a branched-chain amino acid ATP Binding Cassette (ABC) transporter: 1) Mrub_2374 (DNA coordinates 2424832-2425902 on the reverse strand) encodes one permease component (aka transmembrane domain); 2) Mrub_2378 (DNA coordinates 2429525-2430439 on the reverse strand) encodes the second permease component (aka transmembrane domain); 3) Mrub_2376 (DNA coordinates 2427858-2428613 on the reverse strand) encodes one of the ATP-binding domain (aka nucleotide binding domain); 4) Mrub_2377 (DNA coordinates 2428704-2429489 on the reverse strand) encodes a second ATP-binding domain (aka nucleotide binding domain); and 5) Mrub_2379 (DNA coordinates 2430526-2431719 on the reverse strand) encodes the solute or substrate binding protein. The set of genes analyzed are coded for transport of the branched-chain amino acids L-leucine, L-isoleucine, L-valine, and phenylalanine. This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT).
Augustana Digital Commons Citation
Paris, Elizabeth; Steinle, Tony; and Scott, Dr. Lori. "Predicted ortholog pairs between E. coli and M. ruber are b3456 and mrub_2379, b3457 and mrub_2378, b3456 and mrub_2374, b3455 and mrub_2376, and b3454 and mrub2377, which each code for components of a prokaryotic-type ABC transporter for branched-chain amino acids" (2018). Meiothermus ruber Genome Analysis Project.
https://digitalcommons.augustana.edu/biolmruber/37