Course
BIOL-375: Molecular Genetics
Document Type
Student Paper
Publication Date
2018
Disciplines
Biochemistry | Bioinformatics | Biology | Evolution | Genetics | Genomics | Molecular Biology | Molecular Genetics | Other Genetics and Genomics
Description, Abstract, or Artist's Statement
In this project we investigated the biological function of the genes Mrub_1675, Mrub_1676, Mrub_1677, and Mrub_1679 (KEGG map number 02010). We predict these genes encode components of a Branched chain amino acid (ABC) transporter: Mrub_1675 (DNA coordinates 1711022..1712185 on the reverse strand) encodes the permease component, Mrub_1676 (DNA coordinates 1712313..1713170) encodes for the NBD (aka nucleotide binding domain), Mrub_1677 (DNA coordinates 1713167..1714075 on the reverse strand) encodes the NBD (aka nucleotide binding domain), Mrub_1678 (DNA coordinates 1713167..1714075 on the reverse strand) encodes the TMD (aka transmembrane domain) and Mrub_1679 (DNA coordinates 1714781..1715485 on the reverse strand) encodes the cellar component domain. This system is not predicted to have a solute binding protein, which is a component of most ABC transporters. This transport system is found in E. coli K12 MG1655, in Bacillis subtilis the predicted homologs of : Mrub_1675, Mrub_1676, Mrub_1677, Mrub_1678 and Mrub_1679, encode for an ABC transporter for branched chain amino acid. (Cheng et al., 1997). This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT).
Augustana Digital Commons Citation
Patel, Ravi; Hofmann, Alaina; and Scott, Dr. Lori. "Mrub_1675, Mrub_1676, Mrub_1677, and Mrub_1679 genes are orthologs of b_3458, b_3457, b_3456, and b_3454 genes in E. coli, respectively, coding for ABC transporters. Mrub_1678 and b_3455, though perform similar tasks, are not orthologous" (2018). Meiothermus ruber Genome Analysis Project.
https://digitalcommons.augustana.edu/biolmruber/41
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