BIOL-375 Molecular Genetics
Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2994 and Mrub_1844. We predict that Mrub_1884 encodes the enzyme dihydroxy-acid dehydratase (DNA coordinates 1901362..1903026 on the forward strand), which is the third step of the valine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of 2,3-dihydroxy-3methylbutanoate to 3-methyl-2-oxobutanoate. The E. coli K12 MG1655 ortholog is predicted to be b3771, which has the gene identifier ilvD. We also predict that Mrub_2994 encodes the enzyme branched-chain amino acid transaminase. (DNA coordinates 3033210..3034169 on the forward strand), which is the fourth step of the valine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of 3-methyl-2-oxobutanoate to L-valine. The E. coli K12 MG1655 ortholog is predicted to be b3770, which has the gene identifier ilvE.
Augustana Digital Commons Citation
Hartmann, Bennett A. and Scott, Dr. Lori. "Valine Biosynthesis: Mrub_2994 is Orthologous to E. coli b3770 and Mrub_1844 is Orthologous to E. coli b3771" (2016). Meiothermus ruber Genome Analysis Project.