Course

BIOL-375 Molecular Genetics

Document Type

Student Paper

Publication Date

2-2016

Disciplines

Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the gene Mrub_2768 and Mrub_2763. We predict that Mrub_2768 (DNA coordinates 2808186..2809178 on the reverse strand) encodes the enzyme UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (LpxD), which is the third step of the Lipopolysaccharide biosynthesis pathway (KEGG map number 00540). It catalyzes the conversion of UDP-3-O-(3-hydroxymyristoyl)-α-D-glucosamine + a(3R)-3-hydroxymyristoyl-[acp] → a holo-[acyl-carrier protein] + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine. The E. coli K12 MG1655 ortholog is predicted to be b0179, which has the gene identifier LpxD. We also predict that Mrub_2763 (DNA coordinates 2803805..2804974 on the reverse strand) encodes for the enzyme Lipid A disaccharide synthetase (LpxB), which is the fifth step of the Lipopolysaccharide biosynthesis pathway (KEGG map number 00540). It catalyzes the conversion of of 2,3-bis[(3R)-3-hydroxymyristoyol]-α-D-glucosamiyl 1-phosphate + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine → UDP + H + Lipid A disaccharide. Not enough data came up for Mrub_2763 in order to confirm that it was an ortholog of LpxB; the results were inconclusive. The E. coli K12 MG1655 ortholog is predicted to be b0182, which has the gene identifier LpxB.

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