Course

BIOL-375 Molecular Genetics

Document Type

Student Paper

Publication Date

2-2016

Disciplines

Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_0976 and Mrub_1641. We predict that Mrub_0976 encodes the enzyme aspartate kinase (DNA coordinates 964404..965630) which is the 1st step of the homoserine biosynthesispathway (KEGG map number M00018). It catalyzes the conversion L-aspartate to L-asparyl-4-phospate. The E. coli K12 MG1655 ortholog is predicted to be b3940, which has the gene identifier ‘thrA’. We predict that Mrub_1641 encodes the enzyme aspartate-semialdehyde dehydrogenase (DNA coordinates 1672219..1673217) which is the 2nd step of the homoserine biosynthesispathway (KEGG map number M00018). It catalyzes the conversion of L-asparyl-4-phospate to L-aspartate-semialdehyde. The E. coli K12 MG1655 ortholog is predicted to be b3433.

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