Course
BIOL-375 Molecular Genetics
Document Type
Student Paper
Publication Date
2-2016
Disciplines
Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics
Description, Abstract, or Artist's Statement
This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2765 and Mrub_0266. We predict that Mrub_2765 encodes the enzyme β-hydroxyacyl-Acyl carrier protein (ACP) dehydratase (DNA coordinates 2805770..2806213 on the reverse strand), which is the 3rd step of the fatty acid elongation pathway (KEGG map number 00780). It catalyzes the conversion of (3R)-3-hydroxyacyl-[ACP] to trans-2-enoyl-[ACP]. The E. coli K12 MG1655 ortholog is predicted to be b1080, which has the gene identifier FabZ. We also predict that Mrub_0266 encodes the enzyme enoyl-ACP reductase (DNA coordinates 232918..233712 on the reverse strand), which is the 4th step of the fatty acid elongation pathway (KEGG map number 00780). It catalyzes the conversion of trans-2-enoyl-[ACP] to acyl-ACP. The E. coli K12 MG1655 ortholog is predicted to be b1288, which has the gene identifier FabI.
Augustana Digital Commons Citation
Narkis, Amanda M. and Scott, Dr. Lori. "Mrub_2765 is the version of E. coli FabZ in Meiothermus ruber, while Mrub_0266 is the version of E. coli FabI in Meiothermus ruber" (2016). Meiothermus ruber Genome Analysis Project.
https://digitalcommons.augustana.edu/biolmruber/16
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