Course

BIOL-375 Molecular Genetics

Document Type

Student Paper

Publication Date

Winter 2-2016

Disciplines

Bioinformatics | Biology | Genetics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2047 and Mrub_1372. We predict that Mrub_2047 encodes the enzyme fused 4'-phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN-binding (DNA coordinates 2083590..2084816 on the forward strand), which is the first and the second steps of the CoA biosynthesis pathway (KEGG map number 00770). It catalyzes the conversion of (R)-4’-phosphopantothenate to (R)-4’-phosphopantothenoyl-L-cysteine and the conversion of (R)-4’-phosphopantothenoyl-L-cysteine to 4’-phosphopantetheine. The E. coli K12 MG1655 ortholog is predicted to be b3639, which has the gene identifier coaBC. We predict that Mrub_1372 encodes the enzyme pantetheine-phosphate adenylyltransferase (DNA coordinates 1405663..1406160 on the forward strand), which is the third step of the CoA biosynthesis pathway (KEGG map number 00770). It catalyzes the conversion of 4’-phosphopantetheine to Dephospho-CoA. The E. coli K12 MG1655 ortholog is predicted to be b3634, which has the gene identifier coaD.

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