Course

BIOL-375 Molecular Genetics

Document Type

Student Paper

Publication Date

2-2016

Disciplines

Bioinformatics | Biology | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

We predict that Mrub_1905 and Mrub_1906 encode the enzyme 2-isopropylmalate synthase (Mrub_1906 DNA coordinates complement(1965044..1966603) Mrub_1905 DNA coordinates complement(1963455..1965041)), which is the first step of the leucine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of (2S)-2-isopropylmalate to 2-isopropylmaleate. The E. coli K12 MG1655 ortholog is predicted to be b0074, which has the gene identifier leuA. We predict that Mrub_1846 encodes the enzyme 3-isopropylmalate dehydrogenase (DNA coordinates complement(1903909..1904961)), which is the third step of the leucine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of (2R,3S)-3-isopropylmalate to (2S)-2-isopropyl-3-oxosuccinate. The E. coli K12 MG1655 ortholog is predicted to be b0073, which has the gene identifier leuB. We predict that Mrub_1850 encodes for 3-isopropylmalate dehydratase (DNA coordinates complement(1906426..1907850)), the large subunit of the heteromeric enzyme, which is the second step of the leucine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of (2S)-2-isopropylmalate to (2R,3S)-3-isopropylmalate. The E. coli K12 MG1655 ortholog is predicted to be b0072, which has the gene identifier leuC. We predict that Mrub_1847 encodes the enzyme isopropylmalate isomerase (DNA coordinates complement(1904975..1905580)), the small subunit of the heteromeric enzyme, which is the second step of the leucine biosynthesis pathway (KEGG map number 00290). It catalyzes the conversion of (2S)-2-isopropylmalate to (2R,3S)-3-isopropylmalate. The E. coli K12 MG1655 ortholog is predicted to be b0071, which has the gene identifier leuD.

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