Course

BIOL-375: Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

2018

Disciplines

Bacteria | Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

In this project we investigated the biological function of the genes Mrub_1199, Mrub_1200, Mrub_1201, Mrub_2015, Mrub_2271 and Mrub_2272 (KEGG map number 02010). We predict these genes encode components of an Iron (Fe3+) ATP Binding Cassette (ABC) transporter: 1) Mrub_1199 (DNA coordinates [1211595-1212572] on the reverse strand) encodes the permease component (aka transmembrane domain); and 2) Mrub_1200 (DNA coordinates [1212612-1214093] on the reverse strand) encodes the ATP-binding domain (aka nucleotide binding domain); and 3) Mrub_1201 (DNA coordinates [1214347-1215309] on the reverse strand) encodes the substrate binding protein (aka the periplasmic component); and Mrub_2015 ( DNA coordinates [2053963-2054949] on the reverse strand) encodes the substrate binding protein (aka the periplasmic component); and Mrub_2271 (DNA coordinates [2319980-2321551] on the forward strand) encodes the permease component (aka transmembrane domain); and Mrub_2272 (DNA coordinates [2321548-2322639] on the forward strand) encodes the ATP-binding domain (aka nucleotide binding domain). This transport system is found in E. coli K12 MG1655 as the gene afuC (locus tag b0262(DNA coordinates [277756..278802] on the reverse strand) encoding the ABC transporter system for Iron. The afuC gene is the only gene coding for the Iron transport system while M. ruber has genes for all the components. The genes coding for the permease domain and substrate binding protein seem to be absent in E. coli. This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT).

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