Course

BIOL-375: Molecular Genetics

Document Type

Student Paper

Streaming Media

Publication Date

2018

Disciplines

Bacteriology | Bioinformatics | Biology | Evolution | Genetics | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

In this project we investigated the biological function of the genes Mrub_2120, Mrub_2121, Mrub_2122, Mrub_2123 and Mrub_2124 (KEGG map number 02010). We predict these genes encode components of a branched-chain amino acid ATP Binding Cassette (ABC) transporter: 1) Mrub_2120 (DNA coordinates 2169247-2170416 on the reverse strand) encodes the branched-chain amino acid binding protein that is localized to the periplasm; 2) Mrub_2121 (DNA coordinates 2170433..2171353 on the reverse strand) encodes the first TMD; 3) Mrub_2122 (DNA coordinates 2171365..2172279 on the reverse strand) encodes the second TMD; 4) Mrub_2123 (DNA coordinates 2172276..2173028 on the reverse strand) encodes the first NBD; 5) Mrub_2124 (DNA coordinates 2173025…2173735 on the reverse strand) encodes the second NBD. This branched-chain amino acid transport system has been found in E. coli K-12 MG1655 which was used as the model organism in this study. The predicted homologs of Mrub_2120, Mrub_2121, Mrub_2122, Mrub_2123 and Mrub_2124, are livK, livH, livM, livG and livF, respectively. Together, these genes form an operon encoding for an ABC transporter that selectively transports branched-chain amino acids across the intracellular plasma membrane of bacteria. This project is part of the Meiothermus ruber genome analysis project, which predicts gene function using the bioinformatics tools collected under the umbrella of the Guiding Education through Novel Investigation–Annotation Collaboration Toolkit (GENI-ACT).

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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