BIOL-375 Molecular Genetics

Document Type

Student Paper

Publication Date



Bioinformatics | Biology | Genetics | Genetics and Genomics | Genomics | Molecular Biology | Molecular Genetics

Description, Abstract, or Artist's Statement

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. I investigated the biological functions of Mrub_1053 Mrub_2281 and Mrub_2299. I predicted that Mrub_1053 and Mrub_2281 (DNA coordinates 1053364..1054359 on the forward strand and 2333172..2334113 on the forward strand respectively) encodes the enzyme phosphoribose-1-pyrophosphate synthetase (PRS) which is the first step of the purine synthesis pathway (KEGG). I also predicted that Mrub_2299 (DNA coordinates: 2352378..2353775 on the forward strand) encodes for Phosphoribosyl pyrophosphate (PRPP) amidotransferase, which is the second step in this pathway. The E. coli K12 MGI1655 orthologs are predicted to be as follows: Mrub_1053/Mxan_7156, Mrub_2281/Mxan_5075, and Mrub_2299/Mxan_1103.